RNA‐Seq read alignments with PALMapper
Next‐generation sequencing technologies have revolutionized genome and transcriptome
sequencing. RNA‐Seq experiments are able to generate huge amounts of transcriptome
sequence reads at a fraction of the cost of Sanger sequencing. Reads produced by these
technologies are relatively short and error prone. To utilize such reads for transcriptome
reconstruction and gene‐structure identification, one needs to be able to accurately align the
sequence reads over intron boundaries. In this unit, we describe PALMapper, a fast and …
sequencing. RNA‐Seq experiments are able to generate huge amounts of transcriptome
sequence reads at a fraction of the cost of Sanger sequencing. Reads produced by these
technologies are relatively short and error prone. To utilize such reads for transcriptome
reconstruction and gene‐structure identification, one needs to be able to accurately align the
sequence reads over intron boundaries. In this unit, we describe PALMapper, a fast and …