The Drosophila melanogaster transcriptome by paired-end RNA sequencing

B Daines, H Wang, L Wang, Y Li, Y Han… - Genome …, 2011 - genome.cshlp.org
RNA-seq was used to generate an extensive map of the Drosophila melanogaster
transcriptome by broad sampling of 10 developmental stages. In total, 142.2 million uniquely
mapped 64–100-bp paired-end reads were generated on the Illumina GA II yielding 356×
sequencing coverage. More than 95% of FlyBase genes and 90% of splicing junctions were
observed. Modifications to 30% of FlyBase gene models were made by extension of
untranslated regions, inclusion of novel exons, and identification of novel splicing events. A …